Journal: iScience

Article Title: Inhibition of CD44 suppresses the formation of fibrotic scar after spinal cord injury via the JAK2/STAT3 signaling pathway

doi: 10.1016/j.isci.2024.108935

Figure Lengend Snippet: STAT3 was the downstream of JAK2 in CD44-mediated fibrotic effect in the inflammatory microenvironment (A) Western blotting analyzed the expression of p -JAK2, p-STAT3, STAT3, and p -Smad2 in the fibroblasts stimulated with IS after inhibition of AG490. (B‒E) Quantification of western blotting results in A. N = 3 of each group by one-way ANOVA. (F) Western blotting analyzed the expression of CD44, α-SMA, collagen-I, FN, and laminin in the fibroblasts stimulated with IS after inhibition of AG490. (G‒K) Quantification of western blotting results in F. N = 3 of each group by one-way ANOVA. (L and M) Quantification analysis of the rate of proliferation (%) (L) per 10 6 μm 2 (n = 5) and cell numbers of migration (M) per 10 6 μm 2 (n = 5) in different groups by one-way ANOVA. All data were presented as mean ± SEM. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). (See also Figure S3 ).

Article Snippet: AG490 , Targetmol , Cat#T2600;CAS:133550-30-8;Batch:147766.

Techniques: Western Blot, Expressing, Inhibition, Migration

Journal: iScience

Article Title: Inhibition of CD44 suppresses the formation of fibrotic scar after spinal cord injury via the JAK2/STAT3 signaling pathway

doi: 10.1016/j.isci.2024.108935

Figure Lengend Snippet:

Article Snippet: AG490 , Targetmol , Cat#T2600;CAS:133550-30-8;Batch:147766.

Techniques: Recombinant, In Vivo, Lysis, Acid Assay, Software

Journal: Journal of molecular and cellular cardiology

Article Title: Differential hypertrophic effects of cardiotrophin-1 on adult cardiomyocytes from normotensive and spontaneously hypertensive rats.

doi: 10.1016/j.yjmcc.2006.03.433

Figure Lengend Snippet: Fig. 5. Cells were pre-incubated with the indicated chemical inhibitors for 1 h and then with CT-1 for 48 h. Cell area (A), length (B) and width (C) were determined by planimetry. Values represent the mean±standard error of a minimum of 120 cardiomyocytes per experimental condition. Three independent experiments performed in three rats yielded identical results. **p<0.01 vs. baseline; *p<0.05 vs. baseline; $$p<0.01 vs. CT-1; $p<0.05 vs. CT-1. W, Wortmannin; PD1, PD98059 at 10−6 mol/L to inhibit p42/44; PD30, PD98059 at 30×10−6 mol/L to inhibit ERK-5; AG, AG490.

Article Snippet: 10− 6 mol/L to inhibit ERK5 (Calbiochem), the STATs inhibitor AG490 at 10−6 mol/L (Calbiochem), antibodies against LIFR (Santa Cruz) and gp130 (Santa Cruz) at 10−6 mol/L.

Techniques: Incubation

Journal: Journal of molecular and cellular cardiology

Article Title: Differential hypertrophic effects of cardiotrophin-1 on adult cardiomyocytes from normotensive and spontaneously hypertensive rats.

doi: 10.1016/j.yjmcc.2006.03.433

Figure Lengend Snippet: Fig. 6. (A) SHR cardiomyocytes were pre-incubated for 1 h with the STAT3 blocker AG490 and then with CT-1 for 24 h. A representative Western blot and the histogram with bars representing the mean±standard error of three independent experiments are shown for each protein. *p<0.01 vs. baseline and CT-1+AG. AG, AG490 (B) Angiotensinogen mRNA was assayed in cardiomyocytes from Wistar rats and SHR before and after 3 h incubation with CT-1. Bars represent mean±standard error of triplicates in three independent experiments. $p<0.01 vs. baseline; *p<0.01 vs. CT-1.

Article Snippet: 10− 6 mol/L to inhibit ERK5 (Calbiochem), the STATs inhibitor AG490 at 10−6 mol/L (Calbiochem), antibodies against LIFR (Santa Cruz) and gp130 (Santa Cruz) at 10−6 mol/L.

Techniques: Incubation, Western Blot

Journal: PLoS ONE

Article Title: Concurrent Treatment with Taxifolin and Cilostazol on the Lowering of β-Amyloid Accumulation and Neurotoxicity via the Suppression of P-JAK2/P-STAT3/NF-κB/BACE1 Signaling Pathways

doi: 10.1371/journal.pone.0168286

Figure Lengend Snippet: Aa and Ab . Increased intracellular accumulations of Aβ and C-terminal fragment β (C99) in N2a Swe cells. B . Time-dependent increases in the expression of JAK2 phosphorylated at Tyr1007/1008 (P-JAK2) in activated N2a Swe cells. C and D . Concentration-dependent decreases in P-JAK2 expression by taxifolin (10 ~ 50 μM), cilostazol (10 ~ 50 μM), and 20 μM AG490 (a JAK2 inhibitor) determined after culture in medium containing 1% FBS for 3 hr. JAK2 levels showed little change. E . Co-treatment with 10 μM taxifolin plus 10 μM cilostazol significantly decreased P-JAK2 expression. Results are the means ± SEM of 4–5 experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Zero time; ## P < 0.01, ### P < 0.001 vs. Vehicle (Veh); $ $ $ P < 0.001 vs. 10 μM Cilostazol alone; ††† P < 0.001 vs. 10 μM Taxifolin alone.

Article Snippet: AG490 was from Tocris Bioscience (Bristol, UK).

Techniques: Expressing, Concentration Assay

Journal: PLoS ONE

Article Title: Concurrent Treatment with Taxifolin and Cilostazol on the Lowering of β-Amyloid Accumulation and Neurotoxicity via the Suppression of P-JAK2/P-STAT3/NF-κB/BACE1 Signaling Pathways

doi: 10.1371/journal.pone.0168286

Figure Lengend Snippet: Time-dependent changes in the expression of STAT3 phosphorylated at Tyr705 (P-STAT3) in cytosolic ( A ) and nuclear ( B ) fractions of activated N2a Swe cells (3 hr after 1% FBS). C and D . Concentration-dependent decreases in elevated P-STAT3 expression induced by taxifolin (10 ~ 50 μM), cilostazol (10, 30, 50 μM), and 20 μM AG490. E . Co-treatment with 10 μM taxifolin plus 10 μM cilostazol significantly suppressed P-STAT3 expression. Results are the means ± SEMs of 4–7 experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Zero time; ## P < 0.01, ### P < 0.001 vs. Vehicle (Veh); $ $ $ P < 0.001 vs. Cilostazol alone; ††† P < 0.001 vs. 10 μM Taxifolin alone.

Article Snippet: AG490 was from Tocris Bioscience (Bristol, UK).

Techniques: Expressing, Concentration Assay

Journal: PLoS ONE

Article Title: Concurrent Treatment with Taxifolin and Cilostazol on the Lowering of β-Amyloid Accumulation and Neurotoxicity via the Suppression of P-JAK2/P-STAT3/NF-κB/BACE1 Signaling Pathways

doi: 10.1371/journal.pone.0168286

Figure Lengend Snippet: Concentration-dependent increases in IκBα in cytosol ( A ) and decreases in NF-κB in nuclei ( B ) by taxifolin in activated N2a Swe cells (3 hr after 1% FBS) as compared with 20 μM AG490. Co-treatment with 10 μM taxifolin and 10 μM cilostazol significantly increased IκBα levels in cytosol ( C ) and decreased NF-κB levels in nucleus ( Da ), which was confirmed by immunofluorescence study ( Db ). E . Significant decrease in DNA binding activity of NF-κB by co-treatment with 10 μM taxifolin and 10 μM cilostazol as compared with taxifolin or cilostazol monotherapy. Results are the means ± SEMs of 4 experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Vehicle (Veh); $ $ $ P < 0.001 vs. Cilostazol alone; ††† P < 0.001 vs. 10 μM Taxifolin alone; ### P < 0.001 vs. Vehicle (Veh); @@@ P < 0.001 vs. None.

Article Snippet: AG490 was from Tocris Bioscience (Bristol, UK).

Techniques: Concentration Assay, Immunofluorescence, Binding Assay, Activity Assay

Journal: Scientific Reports

Article Title: Activation of M1mAChR’s improves spatial learning and memory deficits in rats exposed to chronic intermittent hypoxia

doi: 10.1038/s41598-025-34689-7

Figure Lengend Snippet: Changes in MWM learning and memory of CIH rats in each group. ( A ) Changes in escape latencies in CIH model rats with AG490 or VU0364572 treatment. The CIH, DMSO, AG490, and AG490 + VU0364572 groups versus the CON group. The VU0364572 group compared with the CIH, DMSO, AG490, and AG490 + VU0364572. F (5,150) = 33.17. All data are presented as mean ± SD ( n = 6). Statistical analysis was performed by one-way ANOVA. *p < 0.05 , ▲ p < 0.05. ( B ) Representative swimming trajectories illustrating search strategies in each group (day 6). ( C,D ) Changes in the number of platforms crossing and time spent in the target quadrant in CIH model rats with AG490 or VU0364572 treatment (day6). The CIH, DMSO, AG490, and AG490 + VU0364572 groups versus the CON group. CIH, DMSO, AG490, and AG490 + VU0364572 group versus the VU0364572 group. All data are presented as mean ± SD ( n = 6). Statistical analysis was performed by one-way ANOVA. *p < 0.05 , ▲ p < 0.05. ( D ) Changes in the number of platforms crossing in CIH model rats with AG490 or VU0364572 treatment. The CIH, DMSO, AG490, and AG490 + VU0364572 groups versus the CON group. CIH, DMSO, AG490, and AG490 + VU0364572 group versus the VU0364572 group. F (5.30) = 24.62. All data are presented as mean ± SD ( n = 6). Statistical analysis was performed by one-way ANOVA. *p < 0.05 , ▲ p < 0.05.

Article Snippet: AG490 (Selleck, USA) and VU0364572 (Cayman, USA) were prepared as DMSO stocks (10 μM) and diluted immediately prior to injection to a final DMSO concentration of 0.1%.

Techniques:

Journal: Scientific Reports

Article Title: Activation of M1mAChR’s improves spatial learning and memory deficits in rats exposed to chronic intermittent hypoxia

doi: 10.1038/s41598-025-34689-7

Figure Lengend Snippet: Observation of pathological changes in hippocampus of CIH rats (40 ×). Note: the picture shows hippocampal neurons in each group of experimental rats. ( A ) CON group. ( B ) CIH group. ( C ) CIH + DMSO group. ( D ) CIH + AG490 group. ( E ) CIH + AG490 + VU0364572 group. ( F ) CIH + VU0364572 group. ( G ) The CIH rat hippocampal CA1 area the number of neurons, *p < 0.05 , compared with the CON group, ▲ p < 0.05 , compared with the VU0364572 group, All the data are expressed in mean ± SD.

Article Snippet: AG490 (Selleck, USA) and VU0364572 (Cayman, USA) were prepared as DMSO stocks (10 μM) and diluted immediately prior to injection to a final DMSO concentration of 0.1%.

Techniques:

Journal: Scientific Reports

Article Title: Activation of M1mAChR’s improves spatial learning and memory deficits in rats exposed to chronic intermittent hypoxia

doi: 10.1038/s41598-025-34689-7

Figure Lengend Snippet: Immunohistochemical analysis of t-STAT3 in hippocampus of CIH rats (40 ×). ( A ) CON group. ( B ) CIH group. ( C ) CIH + DMSO group. ( D ) CIH + AG490 group. ( E ) CIH + AG490 + VU0364572 group. ( F ) CIH + VU0364572 group. (scale = 250 µ m)

Article Snippet: AG490 (Selleck, USA) and VU0364572 (Cayman, USA) were prepared as DMSO stocks (10 μM) and diluted immediately prior to injection to a final DMSO concentration of 0.1%.

Techniques: Immunohistochemical staining

Journal: Scientific Reports

Article Title: Activation of M1mAChR’s improves spatial learning and memory deficits in rats exposed to chronic intermittent hypoxia

doi: 10.1038/s41598-025-34689-7

Figure Lengend Snippet: Immunohistochemical analysis of p-STAT3 in hippocampus of CIH rats (40 ×). ( A ) CON group. ( B ) CIH group. ( C ) CIH + DMSO group. ( D ) CIH + AG490 group. ( E ) CIH + AG490 + VU0364572 group. ( F ) CIH + VU0364572 group. (scale = 250 µ m)

Article Snippet: AG490 (Selleck, USA) and VU0364572 (Cayman, USA) were prepared as DMSO stocks (10 μM) and diluted immediately prior to injection to a final DMSO concentration of 0.1%.

Techniques: Immunohistochemical staining

Journal: Scientific Reports

Article Title: Activation of M1mAChR’s improves spatial learning and memory deficits in rats exposed to chronic intermittent hypoxia

doi: 10.1038/s41598-025-34689-7

Figure Lengend Snippet: Immunohistochemical analysis of M1mAChR in hippocampus of CIH rats (40 ×). ( A ) CON group. ( B ) CIH group. ( C ) CIH + DMSO group. ( D ) CIH + AG490 group. ( E ) CIH + AG490 + VU0364572 group. ( F ) CIH + VU0364572 group; scale = 250 µm)

Article Snippet: AG490 (Selleck, USA) and VU0364572 (Cayman, USA) were prepared as DMSO stocks (10 μM) and diluted immediately prior to injection to a final DMSO concentration of 0.1%.

Techniques: Immunohistochemical staining

Journal: BMC Complementary Medicine and Therapies

Article Title: Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart

doi: 10.1186/s12906-021-03456-2

Figure Lengend Snippet: Experimental protocols. All experimental groups were first perfused for 30 mins on the Langendorff apparatus to allow the isolated hearts to stabilize. The hearts were then divided into different groups. All groups were subjected to 30 mins of regional ischemia followed by 100 mins of reperfusion. IR; Ischemia-reperfusion, IPOST; Ischemic postconditioning (3 × 10 s ischemia and reperfusion at the onset of reperfusion period), Etpost; ethyl acetate fraction from Potentilla reptans root (2 μg/ml) was applied at the onset of reperfusion, L-NAME; NO inhibitor (50 μM), Wort; Wortmannin a PI3K/AKT inhibitor (400 nM), PD; PD98059 (2′-Amino-3′-methoxyflavone) an ERK1/2 inhibitor (400 nM), AG; tyrphostin (AG490, 400 nM) a JAK/STAT3 inhibitor, HD; 5-hydroxy decanoate (5HD,1 μM) a mitoKATP channel blocker

Article Snippet: Wortmannin (Wort; the PI3k/Akt inhibitor), PD98059 (PD; the ERK1/2 inhibitor), Tyrphostin AG490 (the JAK/STAT3 inhibitor), and 5-hydroxy decanoate (a mitoKATP channel blocker) were purchased from “Santa Cruz Biotech, Dallas, Texas, USA”.

Techniques: Isolation

Journal: BMC Complementary Medicine and Therapies

Article Title: Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart

doi: 10.1186/s12906-021-03456-2

Figure Lengend Snippet: Western blot analysis. A Western blot analysis shows the relative intensity of AKT (Tyr473) phosphorylation in heart tissue of IR, Etpost (2 μg/ml) and Etpost+Wort groups that were adjusted relative to GAPDH. B Western blot analysis illustrates the relative intensity of ERK1/2 (Thr183/185) phosphorylation in heart tissue of IR, Etpost (2 μg/ml) and Etpost+PD groups that were adjusted relative to GAPDH. C Western blot analysis displays the relative intensity of STAT3 (Tyr705) phosphorylation in heart tissue of IR, Etpost (2 μg/ml) and Etpost+AG groups that were adjusted relative to GAPDH. The data were expressed as mean ± SEM. * P < 0.05 vs. IR group and # P < 0.05 vs. Etpost (2 μg/ml). Etpost: ethyl acetate fraction of P. reptans root; Wort: Wortmanin (PI3K/Akt inhibitor); PD: PD98059 (ERK inhibitor); AG: AG490 (JAK/STAT3 inhibitor)

Article Snippet: Wortmannin (Wort; the PI3k/Akt inhibitor), PD98059 (PD; the ERK1/2 inhibitor), Tyrphostin AG490 (the JAK/STAT3 inhibitor), and 5-hydroxy decanoate (a mitoKATP channel blocker) were purchased from “Santa Cruz Biotech, Dallas, Texas, USA”.

Techniques: Western Blot, Phospho-proteomics

Journal: Scientific Reports

Article Title: Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action

doi: 10.1038/srep25689

Figure Lengend Snippet: Reagents and Antibodies.

Article Snippet: Tyrphostin AG-490 (19-146) , , Merck S.p.a.(Vimodrone, MI, Italy).

Techniques: Molecular Weight, Recombinant